HPLC MethodAlltesta™ GradientPesticideUV, 275 nm

HPLC Method for Analysis of Carbaryl on Alltesta™ Gradient

Carbaryl, also known as Sevin and 1-naphthyl methylcarbamate, is an insecticide with the chemical formula C12H11NO2. It works as a cholinesterase inhibitor. Carbaryl is highly toxic to zooplankton and works on both targeted and beneficial insects. It is classified as a potential human carcinogen. Carbaryl can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

Chromatogram

Zoom AvailableDouble-tap to reset
Chromatogram for Carbaryl
Loading interactive viewer...
Flow Rate
1ml/min
Injection Volume
1µL
Run Time
6min
Detection
UV, 275 nm
Mobile Phase
M
MeCN
50%
H
H2O
50%
A
AmFm pH 3.0- 20 mM

Frequently Asked Questions: HPLC Method for Analysis of Carbaryl on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Carbaryl on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Carbaryl on Alltesta™ Gradient is designed for Carbaryl, a pesticide compound. Carbaryl, also known as Sevin and 1-naphthyl methylcarbamate, is an insecticide with the chemical formula C12H11NO2.

Which column and mobile phase are required for Carbaryl analysis?

Run the separation on Newcrom B (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU NB-46.150.0510. Typical mobile phase: MeCN, 50% 50% AmFm pH 3.0- 20 mM; H2O 50%.

What instrument conditions should I use for the HPLC Method for Analysis of Carbaryl on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with UV, 275 nm. Flow rate: 1. Injection volume: 1. Run time: 6.

Sample Information

Structure of Carbaryl
Analyte
Carbaryl
Category
Pesticide

Column Information

Newcrom B column

Newcrom B

NB-46.150.0510
Dimensions4.6 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
View Product

Need Method Support?

Our application team can help you adapt this method or develop a new one for your specific needs.

Last updated: Apr 23, 2026

HPLC Method for Analysis of Carbaryl on Alltesta™ Gradient | Applications Library | Newcrom