Chlorpyrifos, also known as Dursban, is a highly toxic organophosphate with the chemical formula C9H11Cl3NO3PS. It works through interrupting the electrochemical processes in nerves. This leads to a build-up of acetylcholine, which leads to paralysis and eventual death. Chlorpyrifos-methyl (Dursban) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
The HPLC Method for Analysis of Chlorpyrifos (Dursban) on Alltesta™ Gradient is designed for Chlorpyrifos (Dursban), a pesticide compound. Chlorpyrifos, also known as Dursban, is a highly toxic organophosphate with the chemical formula C9H11Cl3NO3PS.
Run the separation on Newcrom B (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU NB-46.150.0510. Typical mobile phase: MeCN, 70% 70% AmFm pH 3.0- 20 mM; H2O 30%.
Operate on Alltesta™ Gradient with UV, 275 nm. Flow rate: 1. Injection volume: 1. Run time: 6.
Our application team can help you adapt this method or develop a new one for your specific needs.
Last updated: Apr 23, 2026
