HPLC MethodAlltesta™ GradientHerbicideUV, 275 nm

HPLC Method for Analysis of Propanil on Alltesta™ Gradient

Propanil is a contact herbicide with the chemical formula C9H9Cl2NO. It is one of the most widely used herbicides in America. It works as an inhibitor of photosynthesis and CO2 fixation in weeds. Propanil can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

Chromatogram

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Chromatogram for Propanil
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Flow Rate
1ml/min
Injection Volume
5µL
Run Time
4.5min
Detection
UV, 275 nm
Mobile Phase
M
MeCN
70%
H
H2O
30%
A
AmFm pH 3.0- 20 mM

Frequently Asked Questions: HPLC Method for Analysis of Propanil on Alltesta™ Gradient

What analyte does the HPLC Method for Analysis of Propanil on Alltesta™ Gradient cover?

The HPLC Method for Analysis of Propanil on Alltesta™ Gradient is designed for Propanil, a herbicide compound. Propanil is a contact herbicide with the chemical formula C9H9Cl2NO.

Which column and mobile phase are required for Propanil analysis?

Run the separation on Newcrom B (4.6 x 150 mm, 5 um, 100 A, dual ended), SKU NB-46.150.0510. Typical mobile phase: MeCN, 70% 70% AmFm pH 3.0- 20 mM; H2O 30%.

What instrument conditions should I use for the HPLC Method for Analysis of Propanil on Alltesta™ Gradient?

Operate on Alltesta™ Gradient with UV, 275 nm. Flow rate: 1. Injection volume: 5. Run time: 4.5.

Sample Information

Structure of Propanil
Analyte
Propanil
Category
Herbicide

Column Information

Newcrom B column

Newcrom B

NB-46.150.0510
Dimensions4.6 x 150 mm
Particle5 µm
Pore Size100 Å
Typedual ended
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Last updated: Apr 23, 2026

HPLC Method for Analysis of Propanil on Alltesta™ Gradient | Applications Library | Newcrom